Coding

Part:BBa_K3746003:Design

Designed by: Cheung Chi Ming   Group: iGEM21_Hong_Kong_JSS   (2021-10-17)


FDR-A(MSMEG5998)-6His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a part design for the Phase I project (2021 iGEM). We plan to synthesize the gene fragment by IDT. A 6-His tag is added to the C-terminal for downstream protein purification purpose.

Source

The sequence of MSMEG_5998 (coding sequence of FDR-A) was identified by a literature review [1] and the sequence was then extracted from NCBI (RefSeq: NZ_CP054795.1:4976460-4976939). Codon optimization for E. coli done by ThermoFisher Scientific GeneArt platform.

References

[1] Taylor, M. C., Jackson, C. J., Tattersall, D. B., French, N., Peat, T. S., Newman, J., Briggs, L. J., Lapalikar, G. V., Campbell, P. M., Scott, C., Russell, R. J., & Oakeshott, J. G. (2010). Identification and characterization of two families of F420H2-dependent reductases from mycobacteria that catalyse aflatoxin degradation. Molecular Microbiology, 78(3), 561–575. https://doi.org/10.1111/j.1365-2958.2010.07356.x